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Dietary restriction enhances γ-aminobutyric acid and brain derived neurotrophic factor expression in the visual cortex of the cat
CHEN Cui-yun ZHU Hui LI Peng SUN Qing-yan HUA Tian-miao*
Acta Anatomica Sinica ›› 2014, Vol. 45 ›› Issue (3) : 304-309.
Dietary restriction enhances γ-aminobutyric acid and brain derived neurotrophic factor expression in the visual cortex of the cat
Objective To explore the effect of DR on the expression of inhibitory neurotransmitter, γ-aminobutyric acid (GABA) and brain derived neurotrophic factor (BDNF) in the primary visual cortex of young cats. Methods Totally 6 cats were wsed in this study. Nissl staining was used for cortical layer identification and cell counting. Immunohistochemical techniques were utilized to label GABA- and BDNF-positive neurons. Sections were observed under an Olympus light microscope and photographed with a digital camera. The cell density and absorbance of immunoreactivity were measured with Image-Pro Express 6.0 softwares.
Results Our results showed that the mean density of Nissl-stained neurons in the primary visual cortex (V1) of the DR group showed no significant difference from that of the control group. However, the mean density of GABA-immunoreactive neurons in each cortical layer of V1 in DR cats was significantly higher than that in control cats. The mean immunoreactive intensity of GABA-positive neurons, as indicated by the average absorbance, increased significantly in DR cats relative to control ones. Accompanied with the elevation of GABA expression, DR enhanced BDNF expression in V1, as indicated by an increased mean density of BDNF-positive neurons and BDNF-immunoreactive average absorbance in DR cats relative to controls. Conclusion These results indicate that dietary restriction leads to a concurrent expression upregulation of GABA and BDNF, which may likely compensate for brain functional degradation during senescence and thus delay aging process.
Dietary restriction / γ-Aminobutyric acid / Brain derived neurotrophic factor / Visual cortex / Immunohistochemistry / Cat
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